MB303 (Jan) 3.0 Credits
Elements of Structural Biology
Reference books
Suggested pre-requisite:
Physics/ Chemistry/ Mathematics at Bachelor`s degree level
Course Schedule (January - April)
Lectures 1-10
Topics
General introduction, review of quantum chemistry
Introduction to spectroscopic techniques
Absorption spectroscopy
Circular Dichroism
Fluorescence, anisotropy
11-20
Chromatographic methods
Ultra centrifugation methods
Hydrodynamic techniques, dynamic light scattering
Methods to measure protein-ligand interactions, ITC, DSC
Mass spectrometry
21-30
Crystallography basics
Space group assignments and ambiguities
Strategies in MR
MIR methods
MAD / SAD techniques
Electron density interpretation
Density modification techniques
31-35
X-Ray fiber diffraction
Solution X Ray scattering
Introductory Electron microscopy
Suggested topic s for presentations :
Reference books
- Kensal, E. Van Holde et al., Principles of Physical Biochemistry (Second Edition) Pearson Education International.
- Cantor, C.R., and Schimmel P.R., Biophysical Chemistry, Vols. I-III, W.H. Freeman and Co., San Francisco, USA, 1980
- Research papers and reviews.
Suggested pre-requisite:
Physics/ Chemistry/ Mathematics at Bachelor`s degree level
Course Schedule (January - April)
Lectures 1-10
Topics
General introduction, review of quantum chemistry
Introduction to spectroscopic techniques
Absorption spectroscopy
Circular Dichroism
Fluorescence, anisotropy
11-20
Chromatographic methods
Ultra centrifugation methods
Hydrodynamic techniques, dynamic light scattering
Methods to measure protein-ligand interactions, ITC, DSC
Mass spectrometry
21-30
Crystallography basics
Space group assignments and ambiguities
Strategies in MR
MIR methods
MAD / SAD techniques
Electron density interpretation
Density modification techniques
31-35
X-Ray fiber diffraction
Solution X Ray scattering
Introductory Electron microscopy
Suggested topic s for presentations :
- Peptide sequencing using mass spectrometry
- Experimental methods for protein-ligand interactions
- Protein Dynamics as probed by NMR methods
- Characterization of ion channels
- Features of membrane proteins: structural aspects and protein chemistry
- Fitting high resolution models in low resolution EM maps
